Article, 2024

Pulmonary MicroRNA expression after heterologous challenge with swine influenza A virus (H1N2) in immunized and non-immunized pigs

Virology, ISSN 0042-6822, 1096-0341, Volume 596, Page 110117, 10.1016/j.virol.2024.110117

Contributors

Brogaard, Louise 0000-0003-4571-6822 (Corresponding author) [1] Heegaard, Peter Mikael Helweg 0000-0003-4506-4932 [1] Larsen, Lars Erik 0000-0003-0730-5555 [2] Skovgaard, Kerstin 0000-0001-5663-4879 [1]

Affiliations

  1. [1] Technical University of Denmark
    2. [NORA names: DTU Technical University of Denmark; University; Denmark; Europe, EU; Nordic; OECD];
  2. [2] University of Copenhagen
    3. [NORA names: KU University of Copenhagen; University; Denmark; Europe, EU; Nordic; OECD]

Abstract

MicroRNAs (miRNAs) contribute to post-transcriptional modulation of the host response during influenza A virus (IAV) infection and may be involved in shaping disease severity. Differential disease severity was achieved in two groups of pigs by immunization of one group with a commercial swine IAV vaccine prior to heterologous IAV (H1N2) challenge of both groups. Lung tissue was harvested 1, 3, and 14 days after challenge and miRNA expression was quantified. Gene Ontology term enrichment analysis was employed to examine the functional relevance of genes potentially regulated by differentially expressed miRNAs in pigs with varying degrees of disease severity following IAV infection. Results suggested that the miRNA response associated with less severe disease may modulate host mechanisms essential for viral life cycle, e.g. transcription, translation, and protein trafficking. During more severe disease, miRNA-mediated regulation may focus on dampening virus-specific processes e.g. virion assembly and viral protein processing, and controlling host metabolism.

Keywords

A virus, Gene Ontology term enrichment analysis, H1N2, analysis, assembly, associated with less severe disease, challenges, controlling host metabolism, cycle, days, degree, degree of disease severity, differentiate disease severity, disease, disease severity, enrichment analysis, expressed miRNAs, expression, functional relevance, functional relevance of genes, genes, group, groups of pigs, harvested 1, heterologous challenge, heterologous influenza A viruses, host, host mechanisms, host metabolism, host response, immunity, infection, influenza, influenza A virus, influenza A virus infection, less severe disease, life cycle, lung, lung tissue, mechanism, metabolism, miRNA expression, miRNA response, miRNA-mediated regulation, miRNAs, microRNA expression, microRNAs, modulation, non-immunized pigs, pigs, post-transcriptional modulation, process, processes e., protein processing, regulation, relevance of genes, response, results, severe disease, severity, swine, swine influenza A viruses, tissue, viral life cycle, viral protein processing, virion assembly, virions

Funders

  • Novo Nordisk Foundation

Data Provider: Digital Science

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