open access publication

Article, 2024

Deregulated Long Non-Coding RNAs (lncRNA) as Promising Biomarkers in Hidradenitis Suppurativa

Journal of Clinical Medicine, ISSN 2077-0383, Volume 13, 10, Page 3016, 10.3390/jcm13103016

Contributors

Radhakrishna, Uppala 0000-0002-6617-5634 (Corresponding author) [1] Ratnamala, Uppala [2] Jhala, Devendrasinh D 0000-0002-1339-6492 [2] Uppala, Lavanya V [3] Vedangi, Aaren [4] Saiyed, Nazia [5] Patel, Maulikkumar P 0000-0003-3914-2797 [2] Shah, Sushma R [6] Rawal, Rakesh Mahesh 0000-0002-7985-1187 [2] Jemec, Gregor Borut Ernst 0000-0002-0712-2540 [7] Mazza, Tommaso 0000-0003-0434-8533 [8] Mazzoccoli, Gian Luigi 0000-0003-3535-7635 (Corresponding author) [8] Damiani, Giovanni Battista 0000-0002-2390-6505 (Corresponding author) [9] [10]

Affiliations

  1. [1] University of Pittsburgh
    2. [NORA names: United States; America, North; OECD];
  2. [2] Gujarat University
    3. [NORA names: India; Asia, South];
  3. [3] University of Nebraska at Omaha
    4. [NORA names: United States; America, North; OECD];
  4. [4] Department of Clinical Research, KIMS ICON Hospital, ICON Krishi Institute Medical Sciences, Sheelanagar, Visakhapatnam 530012, India
    5. [NORA names: India; Asia, South];
  5. [5] Department of Obstetrics and Gynecology, Corewell Health William Beaumont University Hospital, Royal Oak, MI 48073, USA
    6. [NORA names: United States; America, North; OECD];

Abstract

Background/Objectives: In recent times, epigenetics alterations in Hidradenitis suppurativa (HS) have been explored and exploited translationally to guide investigation of new therapeutic approaches. On the other hand, long noncoding RNAs (LncRNAs), main regulators of the epigenetic status of the human genome, have been scarcely investigated, notwithstanding their potential relevance in broad pathogenesis comprehension. Here, we aim to explore the methylation pattern of lncRNAs in HS. Methods: In this case-control study, 24 HS patients and age-, sex- and BMI-matched controls were analyzed to characterize the methylome of lncRNA genes in peripheral blood cells. Gene ontology analysis (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis, protein-protein interaction (PPI) network, and MCODE analysis were performed. Results: A set of fifteen lncRNA genes exhibited significantly differential methylation patterns, with ten of them showing hypomethylation and five displaying hypermethylation at specific CpG sites. The hypomethylated lncRNA genes were DLEU2, MESTIT1, CASC2, TUG1, KCNQ1DN, PSORS1C3, PCA3, DSCR8, RFPL1S, and PVT1, while the hypermethylated ones were HAR1A, FAM66B, SNHG9, HCG9, and HCP5. These lncRNA genes have been linked to various important biological processes, including cell proliferation, apoptosis, inflammation, chronic inflammatory skin diseases, and wound healing. Their altered methylation status suggests potential roles in regulating these processes, and may contribute to HS pathogenesis and healing mechanisms. Conclusions: This study revealed an interesting dysregulation pattern of definite lncRNAs in the methylome which is linked to both the development of HS and its comorbidities. Epigenetically altered lncRNAs genes could represent useful biomarkers, and could help in guiding innovative treatment strategies.

Keywords

BMI-matched controls, CpG, CpG sites, Encyclopedia of Genes, Gene ontology analysis, Genomes (KEGG) pathway analysis, HCP5, HS pathogenesis, HS patients, Kyoto, Kyoto Encyclopedia, Kyoto Encyclopedia of Genes, MCODE, MCODE analysis, RNA, age, alterations, analysis, apoptosis, approach, biological processes, biomarkers, blood cells, case-control study, cell proliferation, cells, chronic inflammatory skin disease, comorbidities, comprehension, control, deregulated long non-coding RNAs, deregulation, development, development of HS, disease, dysregulation, dysregulation patterns, epigenetic alterations, epigenetic status, epigenetics, genes, genome, healing, healing mechanisms, hidradenitis, hidradenitis suppurativa, human genome, hypermethylation, hypomethylation, inflammation, inflammatory skin disease, innovative treatment strategies, interaction, investigation, lncRNA genes, lncRNAs, lncRNAs genes, long non-coding RNAs, long noncoding RNAs, mechanism, methylation, methylation patterns, methylation status, methylome, network, non-coding RNAs, noncoding RNAs, ones, ontology analysis, pathogenesis, pathway analysis, patients, patterns, patterns of lncRNAs, peripheral blood cells, process, proliferation, protein-protein interactions, regulation, relevance, sex, sites, skin diseases, status, strategies, study, therapeutic approaches, treatment strategies, wound, wound healing

Data Provider: Digital Science

LINKS
-

Matching Records in NORA

SUBJECTS
+

METRICS
+