Article, 2024

Simultaneous biogas upgrading and single cell protein production using hydrogen oxidizing bacteria

Chemical Engineering Journal, ISSN 1385-8947, 1873-3212, Volume 490, Page 151576, 10.1016/j.cej.2024.151576

Contributors

Li, Rui [1] [2] Jiang, Yufeng 0000-0003-0700-4955 [1] Huang, Jiehua [1] Luo, Kai [1] [2] Fan, Xiao-Lei (Corresponding author) [1] Guo, Rong-Bo [1] Liu, Tao 0000-0001-7673-747X [3] Zhang, Yifeng 0000-0002-2832-2277 [4] Fu, Shan-Fei 0000-0002-5822-5507 (Corresponding author) [1]

Affiliations

  1. [1] Qingdao Institute of Bioenergy and Bioprocess Technology
    2. [NORA names: China; Asia, East];
  2. [2] University of Chinese Academy of Sciences
    3. [NORA names: China; Asia, East];
  3. [3] Hong Kong Polytechnic University
    4. [NORA names: China; Asia, East];
  4. [4] Technical University of Denmark
    5. [NORA names: DTU Technical University of Denmark; University; Denmark; Europe, EU; Nordic; OECD]

Abstract

CO2, a primary byproduct of anaerobic digestion, significantly reduces the calorific value of biogas, necessitating its enhancement through biogas upgrading to increase the CH4 content. The hydrogen oxidizing bacteria (HOB) Cupriavidus necator H16 demonstrates potential as a candidate for biological biogas upgrading due to its efficient CO2 capture and biosynthesis capabilities. Results indicated that in batch experiments, the concentration of CH4 could be elevated from 57.09 % to 98.46 % within 72 h by HOB, with a maximum CO2 assimilation efficiency of 27 mL/(L·h), meeting the requirements for biomethane. In bioreactor scale-up experiments, the CH4 concentration was increased to 94.22 % within 96 h. Beyond biogas upgrading, HOB also produces biomass usable as single cell protein (SCP), with its protein content varying between 43.75–70.83 % depending on the gas supply ratio. A total of 17 amino acids were identified, including eight essential amino acids. The Protein Digestibility Corrected Amino Acid Score (PDCAAS) indicated that the essential amino acid content in the HOB-based protein was well-balanced, closely approximating the quality of fishmeal and pork. A techno-economic analysis revealed that the net revenue from the anaerobic digestion process could be enhanced by 48.49 % using HOB-based biogas upgrading. In contrast, biogas upgrading processes based on hydrogenotrophic methanogens (HM) resulted in a 57 % reduction in net revenue. This study establishes a carbon flow pathway from organic solid waste to biomethane and utilizable protein sources, facilitating sustainable nutrient recovery. This approach not only enhances economic benefits but also reduces carbon emissions associated with the anaerobic digestion process.

Keywords

CH4, CH4 concentrations, CH4 content, CO2, CO2 assimilation efficiency, CO2 capture, Cupriavidus, Cupriavidus necator H16, H16, PDCAAS, acid, acid content, acid score, amino, amino acid content, amino acid score, amino acids, anaerobic digestion, anaerobic digestion process, analysis, assimilation efficiency, bacteria, batch, batch experiments, benefits, biogas, biogas upgrading, biogas upgrading process, biological biogas upgrading, biomass, biomethane, bioreactor, biosynthesis, biosynthesis capability, byproduct of anaerobic digestion, byproducts, calorific value, capability, capture, carbon, carbon emissions, carbon flow pathways, cell protein, cell protein production, concentration, concentrations of CH4, content, digestion, digestion process, economic benefits, efficiency, efficient CO2 capture, emission, enhancement, essential amino acid content, essential amino acids, experiments, fishmeal, flow pathways, gas, hydrogen, hydrogen-oxidizing bacteria, hydrogenotrophic methanogens, methanogens, nutrient recovery, organic solid waste, oxidizing bacteria, pathway, pork, process, production, protein, protein content, protein digestibility, protein production, protein source, quality, quality of fishmeal, ratio, recovery, reduce carbon emissions, reduction, requirements, results, scale-up experiments, scores, simultaneous biogas upgrading, single cell protein, single cell protein production, solid waste, source, study, supply ratio, techno-economic analysis, upgrading, upgrading process, values, waste

Funders

  • National Natural Science Foundation of China
  • Australian Research Council

Data Provider: Digital Science

LINKS
-

Matching Records in NORA

SUBJECTS
+

METRICS
+