open access publication

Article, 2024

Porcine respiratory coronavirus genome sequences; comparisons and relationships to transmissible gastroenteritis viruses

Virology, ISSN 0042-6822, 1096-0341, Volume 595, Page 110072, 10.1016/j.virol.2024.110072

Contributors

Bedsted, Amalie Ehlers [1] Rasmussen, Thomas Bruun 0000-0002-4241-1559 [2] Martinenghi, Laura Daniela [1] [2] Bøtner, Anette G 0000-0002-0558-0222 [1] Nauwynck, Hans J 0000-0001-5470-0713 [3] Belsham, Graham John 0000-0003-1187-4873 (Corresponding author) [1]

Affiliations

  1. [1] University of Copenhagen
  2. [NORA names: KU University of Copenhagen; University; Denmark; Europe, EU; Nordic; OECD];
  3. [2] Statens Serum Institut
  4. [NORA names: SSI Statens Serum Institut; Governmental Institutions; Denmark; Europe, EU; Nordic; OECD];
  5. [3] Ghent University
  6. [NORA names: Belgium; Europe, EU; OECD]

Abstract

Porcine respiratory coronavirus (PRCV) was initially detected in Europe, and later in the United States of America (US), in the 1980s. In this study we obtained and compared PRCV sequences from Europe and the US, and investigated how these are related to transmissible gastroenteritis virus (TGEV) sequences. The whole genome sequences of Danish (1/90-DK), Italian (PRCV15087/12 III NPTV Parma), and Belgian PRCV (91V44) strains are presented. These sequences were aligned with nine other PRCV sequences from Europe and the US, and 43 TGEV sequences. Following alignment of the PRCV sequences, it was apparent that multiple amino acid variations in the structural proteins were distinct between the European and US strains. The alignments were used to build phylogenetic trees to infer the evolutionary relationships between the strains. In these trees, the European PRCV strains clustered as a separate group, whereas the US strains of PRCV all clustered with TGEVs.

Keywords

America, Europe, States of America, TGEV, US strains, United States, United States of America, acid variations, alignment, amino acid variations, coronavirus, evolutionary relationships, gastroenteritis, gastroenteritis virus, group, multiple amino acid variations, phylogenetic tree, porcine, porcine respiratory coronavirus, protein, relationship, respiratory coronavirus, sequence, strain, structural proteins, study, trees, units, variation, virus

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