Article, 2024

Selective colonization of multifunctional microbes that facilitates caproate production in microbial electrosynthesis system

Chemical Engineering Journal, ISSN 1385-8947, 1873-3212, Volume 488, Page 150848, 10.1016/j.cej.2024.150848

Contributors

Wang, Yuru [1] [2] Cheng, Shuanglan [3] Varrone, Cristiano 0000-0002-5638-6390 [4] Liu, Zhihong 0000-0002-2372-2490 [1] He, Zhang-Wei [5] Zhou, Ai-Juan 0000-0002-8116-0297 (Corresponding author) [1] Yue, Xiu-Ping [1] [2] Wang, Ai-Jie [6] Liu, Wen-Zong 0000-0002-1331-6480 [6]

Affiliations

  1. [1] Taiyuan University of Technology
    2. [NORA names: China; Asia, East];
  2. [2] Shanxi-Zheda Institute of Advanced Materials and Chemical Engineering, Taiyuan 030024, China
    3. [NORA names: China; Asia, East];
  3. [3] Donghua University
    4. [NORA names: China; Asia, East];
  4. [4] Aalborg University
    5. [NORA names: AAU Aalborg University; University; Denmark; Europe, EU; Nordic; OECD];
  5. [5] Xi'an University of Architecture and Technology
    6. [NORA names: China; Asia, East];

Abstract

As promising and value-added platform chemicals, the biosynthesis of medium-chain fatty acids (MCFAs, C6-C12) via chain elongation (CE) in microbial electrosynthesis systems (MES) has recently garnered significant attentions due to the unique bioelectrocatalytic behaviors and the reduction of exogenous electron donors (EDs). Caproate-synthesizing bacteria (CSB) played a crucial role in the generation of acetyl-CoA, which subsequently underwent reverse β oxidation to synthesize MCFAs. However, the presence of electrochemically active bacteria (EAB) was also pivotal for MES as they generated hydrogen in the cathodic electron transfer pathways, the specific colonization patterns of key symbionts remained unknown. The aim of this study was to investigate the selective colonization of EAB and CSB on the performance of caproate production in MES. The reactors colonized EAB and CSB achieved the maximum caproate concentration of 5332.5 mg COD/L, which was 7.9 and 1.3 times higher compared to that inoculated with either sole EAB or CSB, respectively. Moreover, the reactors colonized with mixed culture exhibited a clear redox peak and preferable electron transfer efficiency. CSB and EAB mutually interacted in metabolic activities, thereby resulting in increased substrate utilization and caproate production. The findings of this study provide a new strategy and insights for value-added chemicals recovery in microbial electrosynthesis platform.

Keywords

B oxidation, COD/L, MCFA, acetyl-CoA, acid, active bacteria, activity, bacteria, behavior, biosynthesis, biosynthesis of medium-chain fatty acids, caproate, caproate concentration, caproate production, chain, chain elongation, chemical, chemical recovery, colon, colonization patterns, concentration, donor, efficiency, electrochemically active bacteria, electron, electron donor, electron transfer efficiency, electron transfer pathway, elongation, exogenous electron donor, fatty acids, findings, generation, generation of acetyl-CoA, hydrogen, increased substrate utilization, medium-chain fatty acids, metabolic activity, microbes, microbial electrosynthesis system, pathway, patterns, peak, performance, platform, platform chemicals, presence, production, reactor, recovery, redox, redox peaks, reduction, study, substrate utilization, symbionts, system, transfer efficiency, transfer pathway, utilization, value-added platform chemicals

Funders

  • National Natural Science Foundation of China

Data Provider: Digital Science

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