open access publication

Article, 2024

From microbial heterogeneity to evolutionary insights: A strain-resolved metagenomic study of H2S-induced changes in anaerobic biofilms

Chemical Engineering Journal, ISSN 1385-8947, 1873-3212, Volume 485, Page 149824, 10.1016/j.cej.2024.149824

Contributors

Ghiotto, Gabriele 0009-0000-6600-4272 [1] De Bernardini, Nicola 0000-0003-3887-737X [1] Giangeri, Ginevra 0000-0002-5944-6811 [2] Tsapekos, Panagiotis 0000-0002-5309-4624 [2] Gaspari, Maria 0000-0002-8654-1144 [3] Kougias, Panagiotis G 0000-0003-4416-2135 [3] Campanaro, Stefano 0000-0002-9431-1648 (Corresponding author) [1] Angelidaki, Irini 0000-0002-6357-578X [2] Treu, Laura 0000-0002-5053-4452 [1]

Affiliations

  1. [1] University of Padua
    2. [NORA names: Italy; Europe, EU; OECD];
  2. [2] Technical University of Denmark
    3. [NORA names: DTU Technical University of Denmark; University; Denmark; Europe, EU; Nordic; OECD];
  3. [3] Soil and Water Resources Institute, Hellenic Agricultural Organization - Dimitra, Thermi, Thessaloniki 57001, Greece
    4. [NORA names: Greece; Europe, EU; OECD]

Abstract

The hidden layers of genomic diversity in microbiota of biotechnological interest have been only partially explored and a deeper investigation that overcome species level resolution is needed. CO2-fixating microbiota are prone to such evaluation as case study. A lab-scale trickle-bed reactor was employed to successfully achieve simultaneous biomethanation and desulfurization on artificial biogas and sulfur-rich biogas, and oxygen supplementation was also implemented. Under microaerophilic conditions, hydrogen sulfide removal efficiency of 81% and methane content of 95% were achieved. Methanobacterium sp. DTU45 emerged as predominant, and its metabolic function was tied to community-wide dynamics in sulfur catabolism. Genomic evolution was investigated in Gammaproteobacteria sp. DTU53, identified as the main contributor to microaerophilic desulfurization. Positive selection of variants in the hydrogen sulfide oxidation pathway was discovered and amino acid variants were localized on the sulfide entrance channel for sulfide:quinone oxidoreductase. Upon oxygen supplementation strain selection was the primary mechanism driving microbial adaptation, rather than a shift in species dominance. Selective pressure determined the emergence of new strains for example on Gammaproteobacteria sp. DTU53, providing in depth evidence of functional redundancy within the microbiome.

Keywords

Gammaproteobacteria, acid variants, adaptation, amino, amino acid variants, anaerobic biofilms, artificial biogas, biofilm, biogas, biomethane, biotechnological interest, case study, cases, catabolism, changes, channel, conditions, content, desulfurization, diversity, dominance, dynamics, efficiency, emergency, entrance channel, evaluation, evidence, evidence of functional redundancy, evolution, evolutionary insights, function, functional redundancy, genome evolution, genomic diversity, hidden layer, hydrogen, hydrogen sulfide oxidation pathway, hydrogen sulfide removal efficiency, insights, interest, investigation, level resolution, mechanism, metabolic functions, methane, methane content, microaerophilic conditions, microbial adaptation, microbiome, microbiota, oxidation pathway, oxygen, oxygen supplementation, pathway, positive selection, pressure, primary mechanism, reactor, redundancy, removal efficiency, resolution, selection, selection of variants, selection pressure, shift, species, species dominance, species level resolution, strain, strain selection, study, sulfide, sulfide oxidation pathway, sulfide removal efficiency, sulfur, sulfur catabolism, supplementation, trickle-bed reactor, variants

Funders

  • European Commission

Data Provider: Digital Science

LINKS
-

Matching Records in NORA

SUBJECTS
+

METRICS
+