Article,
Dilarmycins A–C, Calcium-Dependent Lipopeptide Antibiotics with a Non-canonical Ca2+-Binding Motif
Contributors
Fernandez-Pastor, Ignacio
0000-0003-1729-0860
[1]
Ortiz-López, Francisco Javier
0000-0002-9410-3718
(Corresponding author)
[1]
Oves-Costales, Daniel
0000-0001-9986-4623
(Corresponding author)
[1]
Martín, Jesús
0000-0001-7487-2790
[1]
Reyes, Fernando
0000-0003-1607-5106
[1]
Weber, Tilmann
0000-0002-8260-5120
[2]
Genilloud, Olga
0000-0002-4202-1219
[1]
Affiliations
- [1] Fundación Medina [NORA names: Spain; Europe, EU; OECD];
- [2] Technical University of Denmark [NORA names: DTU Technical University of Denmark; University; Denmark; Europe, EU; Nordic; OECD]
Abstract
Genome analysis of strain Streptomyces sp. CA-278952 revealed a biosynthetic gene cluster encoding a putative lipopeptide with a sequence containing an Asp-Gly-Glu-Ala motif. We envisioned that this motif could mimic the canonical Asp-X-Asp-Gly sequence found in previously reported calcium-dependent lipopeptide antibiotics. Chemical investigation of the producing strain led to the discovery of three novel lipodepsipeptides, dilarmycins A-C. The calcium-dependent antibacterial activity of the new compounds was confirmed against the Gram-positive pathogens methicillin-resistant Staphylococcus aureus and vancomycin-resistant Enterococcus.
Keywords
A-C.,
Asp-Gly-Glu-Ala,
Ca2+-binding motif,
a-C,
activity,
antibacterial activity,
antibiotics,
binding motif,
biosynthetic gene cluster,
calcium-dependent lipopeptide antibiotics,
chemical,
chemical investigation,
clusters,
compounds,
discovery,
gene cluster,
genome,
genomic analysis,
investigation,
lipodepsipeptides,
lipopeptide antibiotic,
lipopeptides,
methicillin-resistant <i>Staphylococcus aureus</i> (MR,
motif,
sequence,
strain,
vancomycin-resistant <i>En
