open access publication

Article, 2024

Identification of Clubroot-Resistant Germplasm in a Radish (Raphanus sativus L.) Core Collection

Agronomy, ISSN 2073-4395, Volume 14, 1, Page 157, 10.3390/agronomy14010157

Contributors

Ma, Yang [1] [2] Wang, Haiping 0000-0002-3056-1656 [1] Song, Jiangping [1] Yang, Wenlong 0000-0003-2387-9559 [1] Jia, Hui-Xia 0000-0002-5517-5698 [1] Agerbirk, Niels 0000-0003-4670-8330 [3] Chen, Yinan [1] Li, Chen [1] [2] Piao, Yinglan [1] Li, Sen [2] Zhang, Xiaohui 0000-0001-9610-854X (Corresponding author) [1]

Affiliations

  1. [1] Institute of Vegetables and Flowers
    2. [NORA names: China; Asia, East];
  2. [2] Shanxi Agricultural University
    3. [NORA names: China; Asia, East];
  3. [3] University of Copenhagen
    4. [NORA names: KU University of Copenhagen; University; Denmark; Europe, EU; Nordic; OECD]

Abstract

Clubroot disease, caused by Plasmodiophora brassicae, poses a significant global threat to cruciferous crops. The epidemic area of clubroot disease is expanding rapidly. In response to this pressing issue, there is a compelling need for the development of clubroot disease-resistant radish cultivars. China boasts an extensive array of radish varieties and germplasm resources. However, a comprehensive assessment of their resistance to clubroot has not yet been carried out, thereby impeding the effective utilization of germplasm and clubroot-resistant breeding. Therefore, it is urgent to systematically evaluate the clubroot resistance of the radish germplasm and identify resistant resources. In this study, clubroot resistance evaluations were conducted on 268 excellent radish varieties derived from 30 provinces in China, as well as seven accessions from Russia, North Korea, France, South Korea, and Germany. The resistance evaluation revealed a diverse range of resistance indices, with a mean disease index (DI) ranging from 0.6 to 58.5, showing significant disparities in clubroot resistance among these radish resources. A total of six accessions were characterized as highly resistant to clubroot, and a further 50 accessions were characterized as resistant. The disease-resistant radishes showed diversity in horticultural traits. Provinces in South China contributed significantly more resistance germplasm than those of North China. These materials are of great value for both genetic investigation and the crop breeding of clubroot resistance. Furthermore, we employed a previously established clubroot-resistance-linked SSR marker to analyze the clubroot-resistant resources. The accessions exhibited dissimilar genetic profiles from known clubroot-resistant germplasm, suggesting their potential status as novel sources of clubroot resistance. Conclusively, these newly identified accessions enriched the genetic diversity within the clubroot-resistant gene pool and may contribute to the future cloning of previously undiscovered clubroot-resistant genes.

Keywords

Brassica, China, France, Germany, Korea, North, North China, North Korea, Plasmodiophora, Plasmodiophora brassicae, Russia, SSR, SSR markers, South, South China, South Korea, access, area, array, assessment, breeding, clones, clubroot, clubroot disease, clubroot resistance, clubroot resistance genes, collection, comprehensive assessment, core collection, crop, crop breeding, cruciferous crops, cultivars, development, disease, disease index, disparities, diversity, effective utilization, epidemic areas, evaluation, gene pool, genes, genetic diversity, genetic investigations, genetic profile, germplasm, germplasm resources, global threat, horticultural traits, identification, index, investigation, issues, markers, materials, mean disease index, pool, potential status, profile, province, radish, radish cultivars, radish germplasm, radish varieties, resistance, resistance evaluation, resistance index, resistance resources, resistance to clubroot, resistant germplasm, resources, response, source, sources of clubroot resistance, status, study, threat, traits, utilization of germplasm, varieties

Funders

  • National Natural Science Foundation of China
  • Chinese Academy of Agricultural Sciences

Data Provider: Digital Science

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