Article, 2023

Novel Chip for Applying Mechanical Forces on Human Skin Models Under Dynamic Culture Conditions

Tissue Engineering Part C Methods, ISSN 1937-3392, 1937-3384, Volume 30, 2, Pages 85-91, 10.1089/ten.tec.2023.0195

Contributors

Kaiser, Katharina 0000-0003-0399-3899 [1] Sørensen, Jens A H M 0000-0003-4903-0094 [2] Brewer, Jonathan Richard 0000-0002-3444-1715 (Corresponding author) [1]

Affiliations

  1. [1] University of Southern Denmark
    2. [NORA names: SDU University of Southern Denmark; University; Denmark; Europe, EU; Nordic; OECD];
  2. [2] Odense University Hospital
    3. [NORA names: Region of Southern Denmark; Hospital; Denmark; Europe, EU; Nordic; OECD]

Abstract

In recent years the need for in vitro skin models as a replacement for animal studies has resulted in significant progress in the development of skin-on-a-chip models. These devices allow the fine control of the microenvironment of the model and the incorporation of chemical and physical stimuli. In this study, we describe the development of an easy and low-budget open-top dynamic microfluidic device for skin-on-a-chip experiments using polydimethylsiloxane and a porous polyethylene terephthalate membrane. The chip allows the incorporation of compressive stimuli during the cultivation period by the use of syringe pumps. Proof-of-concept results show the successful differentiation of the cells and establishment of the skin structure in the chip. The microfluidic skin-on-a-chip models presented in this study can serve as a platform for future drug and feasibility studies.

Keywords

Proof-of-concept results, animal studies, animals, cells, chemical, chip, compressive stimuli, conditions, control, cultivation, cultivation period, culture conditions, development, devices, differentiation, drug, dynamic culture conditions, dynamic microfluidic device, dynamics, establishment, experiments, feasibility, feasibility study, force, future drugs, human skin model, humans, incorporation, incorporation of chemical, mechanical forces, mechanism, membrane, microenvironment, microfluidic devices, model, novel chip, period, physical stimuli, platform, polydimethylsiloxane, polyethylene terephthalate membranes, progression, proof-of-concept, pump, replacement, results, skin, skin model, skin structure, skin-on-a-chip, skin-on-a-chip model, stimuli, structure, study, syringe, syringe pump, years

Funders

  • Novo Nordisk Foundation

Data Provider: Digital Science

LINKS
-

Matching Records in NORA

SUBJECTS
+

METRICS
+