open access publication

Article, 2024

Profile control and plugging ability of extracellular polymeric substances (EPS) produced by E. cloacae strain ZL-02 under Ba2+ in reservoir

Fuel, ISSN 0016-2361, 1873-7153, Volume 355, Page 129453, 10.1016/j.fuel.2023.129453

Contributors

Lin, Xiaosha [1] [2] [3] Zheng, Xuecheng [1] [2] Yu, Haoshui 0000-0002-9256-852X [3] Li, Dong Wei (Corresponding author) [2]

Affiliations

  1. [1] Southwest Petroleum University
    2. [NORA names: China; Asia, East];
  2. [2] Chongqing University
    3. [NORA names: China; Asia, East];
  3. [3] Aalborg University
    4. [NORA names: AAU Aalborg University; University; Denmark; Europe, EU; Nordic; OECD]

Abstract

Microbial Enhanced Oil Recovery (MEOR) is a pivotal technology for enhancing tertiary oil recovery. The production of partly extracellular polymeric substances (EPS) by Enterobacter cloacae exhibits excellent control over mobility and holds promise for applications in heterogeneous reservoir exploration. To investigate the impact of Ba2+ on microbial EPS production, a series of tests were conducted, including EPS production tests, polysaccharide and protein content analyses, Inductively Coupled Plasma Optical Emission Spectrometer (ICP-OES) measurements, X-ray diffraction (XRD), laser particle size measurements, and transcriptome analysis.The results demonstrated the stimulating effect of Ba2+ on EPS membrane production. Ba2+ stimulation led to increased secretion of proteins (5.82 g/L) and polysaccharides (9.0 g/L). Moreover, the binding of proteins with Ba2+ resulted in an enlargement of the particle size of the EPS membrane and an increase in grain size, reaching a maximum crystallinity of 77.77 %. Transcriptome analysis revealed the up-regulation of 790 genes and down-regulation of 728 genes in response to Ba2+ addition. Enrichment analysis of the up-regulated genes highlighted the significant enrichment of gene ontology number GO: 00160120, which directly influenced microbial EPS membrane production. Furthermore, microscopic displacement and core flooding experiments were conducted to evaluate the plugging ability of microbial-produced EPS membranes. Remarkably, under the influence of Ba2+, microorganisms exhibited significantly enhanced sealing capacity on glass slides etched with rock cores. The results from simulated flow experiments demonstrated a maximum sealing rate of 81.5 % on rock cores, with an RF (Resistance Factor) value of 2.5 and an RRF (Residual Resistance Factor) value of 5.45. This study innovatively explores oil-producing microbial genes, combining simulation experiments and rock core slicing to reveal the microscale mechanism of Ba2+ stimulating microbial EPS secretion for reservoir plugging. It provides theoretical support for the application of MEOR technology in improving oil recovery rates.

Keywords

Ba2, Ba2+, EPS production, EPS secretion, Enterobacter cloacae, ICP-OES, Inductively Coupled Plasma Optical Emission Spectrometer (ICP-OES, RF, RRF, X-ray, X-ray diffraction, ability, analysis, applications, binding, binding of proteins, capacity, content analysis, control, core, core flooding experiments, crystallinity, diffraction, displacement, down-regulation, effect of Ba2, enlargement, experiments, exploration, extracellular polymeric substances, factors, flooding experiments, flow experiments, genes, glass, glass slides, grain, grain size, impact, improve oil recovery rate, increase, increased secretion, increased secretion of proteins, induction, influence, laser, laser particle size measurement, maximum crystallinity, measurements, membrane, membrane production, microbial EPS production, microbial enhanced oil recovery, microbial genes, microorganisms, microscale mechanisms, microscopic displacement, mobility, oil recovery, oil recovery rate, particle size, particle size measurements, particles, plug, plugging ability, polymeric substances, polysaccharides, production, production test, profile control, protein, protein content analysis, rate, recovery, recovery rate, reservoir, reservoir exploration, reservoir plugging, resistance, resistance factor, response, results, rock cores, rocks, seal, sealing capacity, sealing rate, secretion, secretion of proteins, significant enrichment, simulated flow experiments, simulation, simulation experiments, size, size measurements, slides, stimulating effect, stimulation, study, substances, support, technology, tertiary oil recovery, test, theoretical support, transcriptome, transcriptome analysis, up-regulated, up-regulated genes

Funders

  • National Natural Science Foundation of China
  • Science and Technology Department of Sichuan Province

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