Article,
A 3D printable synthetic hydrogel as an immobilization matrix for continuous synthesis with fungal peroxygenases
Contributors
Meyer, Lars-Erik
0000-0003-2591-0118
[1]
Vaupel, Sonja
0000-0003-3536-5774
[2]
Meyer, Johanna
0000-0003-2557-4425
[2]
Alcalde, M Isabel
0000-0001-6780-7616
[3]
Kara, Selin
0000-0001-6754-2814
[1]
[2]
Affiliations
- [1] Aarhus University [NORA names: AU Aarhus University; University; Denmark; Europe, EU; Nordic; OECD];
- [2] Leibniz University Hannover [NORA names: Germany; Europe, EU; OECD];
- [3] Instituto de Catálisis y Petroleoquímica [NORA names: Spain; Europe, EU; OECD]
Abstract
Development of a novel immobilization strategy using synthetic 3D printable hydrogels for flow biocatalysis. Enzyme immobilization is the key to an intensified bioprocess that allows recycling of the heterogenized enzyme and/or continuous biocatalytic production. In this communication, we present a case study for enzyme immobilization in a novel, 3D printable synthetic hydrogel and its use in continuous oxidation reactions. Immobilization resulted in an average immobilization yield of 6.1% and continuous synthesis was run for 24 hours with a space–time yield of 3.1 × 10 −2 g L −1 h −1 .
Keywords
biocatalysis,
biocatalytic production,
bioprocess,
case study,
cases,
communication,
continuous synthesis,
development,
enzyme,
enzyme immobilization,
flow,
flow biocatalysis,
fungal peroxygenases,
heterogeneous enzyme,
hydrogels,
immobilization,
immobilization matrix,
immobilization strategy,
immobilization yield,
matrix,
novel immobilization strategy,
oxidation reaction,
peroxygenase,
printable hydrogels,
production,
reaction,
recycling,
space-time yield,
strategies,
study,
synthesis,
synthetic hydrogels,
yield
