PERSONALIZED CANCER THERAPY TARGETING NORMALLY NON-EXPRESSED SEQUENCES

Provided is a method for identifying immunogenic amino acid sequences in a sample of malignant tissue from a patient comprising A) determining amino acid sequences of proteinaceous expression products from the malignant tissue,B) analysing said amino acid sequences to identify therein proteinaceous expression products of selected genomic sequences in the patient's species, C) identifying - in the proteinaceous expression products – the amino acid sequences, which are those that will bind to MHC molecules of the patient,where said selected genomic sequences constitute a subset of all sequences of the genome of said species and where said subset is constituted by sequences, which encode proteinaceous expression products in at most 5% of samples from any healthy tissue, where said healthy tissue is a tissue of a type found in the patient, and where said healthy tissue optionally does not include testis tissue and/or brain tissue. Also, a related method for treatment of cancer is provided as well as a computer system.In addition is provided a method for stratifying cancer patients into groups of those eligible for immunotherapy or not, based on their burden of expression of endogenous retrovirus.